HTSF - Analyst HT

Instructions on using the Analyst HT machine.

Before turning on the instrument:

For luminescence reading only: Skip steps 1 through 10 if using fluorescence.

  1.  Check if the indicator for setting of Analyst HT luminescence is 96 or 384.
    • Picture1AHT.png
  2. If it does NOT need to be changed, turn on the power and skip steps 3-10. If it needs to be changed, unplug the power and continue. 
    • Picture2AHT.png
  3. Take off the metal side panel by pulling bottom ridge.
    • Picture3AHT.png
  4. Turn counter-clock wise to unscrew the two metal screws.
    • Picture4AHT.png
  5.  Take off the black seal panel and put on benchtop, so the inside chamber is revealed.
    • Picture5AHT.png
    • Picture6AHT.png
  6. Follow the instruction to push the button in for 96 wells, or pull out for 384 wells.
    • Picture7AHT.png
    • Picture8AHT.png
  7.  Put the black panel back and tighten the two screws.
  8. Put metal side panel back.
  9. Change the 96 or 384 indicator into the correct position.
  10.  Put power cord back and turn on instrument. Go to Step 26. Before turning on the instrument: For fluorescence reading only. For Luminescence, go to step 26 for reading after step 10. 
  11. Check the indicator about which Dichroic Mirror is inside the plate reader. Your application is ___________
    • Picture9AHT.png
  12.  If it does not need to be changed, turn on the power. If it needs to be changed, unplug the power and continue. 
    • Picture10AHT.png
  13.  Take off the metal side panel by pulling bottom ridge.
    • Picture11AHT.png
  14. Turn counter-clock wise to unscrew the two metal screws.
    • Picture12AHT.png
  15. Take off the black seal panel and put on benchtop, so the inside chamber is revealed.
    • Picture13AHT.png
    • Picture14AHT.png
  16. Open drawer below the instrument marked with “Analyst HT”. Open the empty box, matching the wavelength to the one inside the plate reader.
  17. Wear a pair of new gloves to keep clean. Always avoid touching the mirror surface.
  18. Pull the Dichroic Mirror out as shown.
    • Picture15AHT.png
    • Picture16AHT.png
    • Picture17AHT.png
  19.  Put the Dichroic Mirror back to the correct box. Make sure to secure the ring in the slot.
    • Picture18AHT.png
  20.  Open the box with the Dichroic Mirror your application need _____________ nM.
  21. Face the round side of the Dichroic Mirror up right and flat side bottom left, slide it inside the slot.
    • Picture19AHT.png
  22. Put the black panel back and tighten the two screws. 
  23. Put metal side panel back.
  24. Change the Dichroic Mirror indicator into the correct position.
  25. Put power cord back and turn on instrument. 
  26. After instrument turn on:  Wait for the plate reader finish self-calibration. The instrument screen will show “LJL Biosystems” and the drawer should come out.
    • Picture20AHT.png
  27.  Log in and open the control software “CriterionHost”. 
    • Picture21AHT.png
  28. Check the status bar at the bottom right corner. Everything is ok if it is green. If it is black, it indicates the software not communicating with the instrument, close the software and reopen it after plate reader self-calibration is completely done.  If it is still black, restart the computer. If it is red, there is error with the instrument, turn the plate reader off and back on. If it is still red, contact staff.
    • Picture22CompleteAHT.png
  29. Select your reading protocol type and name.  My protocol type___________ Name_____________
    • Picture24AHT.pngPicture25AHT.png
  30. Go the “method”>>”edit”, or click the pencil icon.
    • Picture26AHT.png
  31. Click “Select wells”.
    • Picture27AHT.png
  32. Use “clear all” to clear previous selected wells and drag to select wells to be measured, then click “OK”.
    • Picture28AHT.png
  33. If kinetics need to be measured, Click “Advanced”.  If not, go to step 35.
    • Picture29AHT.png
  34.  Input “number of reads” (maximum 100) and “delay between reads” (minimal interval limited by how long it takes to read all selected wells once). Then click “OK”.
    • Picture30AHT.png
  35. Click “OK” on method edit interface.
    • Picture31AHT.png
  36. Go to “File”>> “Save as” to save the file before reading. 
    • Picture32AHT.png 
  37.  Open the drawer cover, the drawer should be out for loading the plate. Go to step 40 if looks fine.
    • Picture33AHT.png
  38.  If the drawer is not out, check if the stacker is in use. If the icon is embedded, click it and wait for the instrument to adjust to manual feeding mode and the drawer to come out.
    • Picture34AHT.png
  39. If the drawer still does not come out, restart the instrument. If the problem persists, contact staff.  
  40. Take off the cover of the drawer and put plate inside (A1 is face the top left indicator).
    • Picture35AHTEdited.png
  41. Double check the direction of plate to make sure A1 is facing the correct position, and the plate is located within the rims. It is alright if the plate can wiggle around a little; it will be secured after the plate is taken inside for reading.
    • Picture36AHTEdited.png
  42. Put the cover back on the drawer.
    • Picture37AHTEdited.png
  43. Click “read”.
    • Picture38AHT.png
  44. If more than one plate need to be read, replace the plate and click read again. The data will be automatically saved to the same file.  
  45. After done, close software and log out.
  46. Take plate out of the instrument and power off the plate reader. 
  47. The saved file can be opened with excel.  







Keywords:analyst ht, analyst, ht, scs_cores, scs, cores, htsf   Doc ID:111166
Owner:Vishnu K.Group:University of Illinois School of Chemical Sciences
Created:2021-06-02 22:07 CDTUpdated:2022-01-21 19:28 CDT
Sites:University of Illinois School of Chemical Sciences
CleanURL:https://answers.uillinois.edu/analyst-ht
Feedback:  0   0